Antimicrobial resistance of Staphylococcus aureus and Pseudomonas spp. isolated from coated skewers sold in Ouagadougou, Burkina Faso

Abstract

Coated skewers are very popular in Ouagadougou. This work aims to assess the microbial quality ofcoated skewers sold in the streets of Ouagadougou and their isolated Staphylococcus aureus andPseudomonas spp antimicrobial resistance. A total of 30 coated skewers samples were collectedfrom various processors for microbial analysis using standard methods. The antimicrobialresistance test was performed using the agar plate diffusion method. The microbial load variedfrom 6.0×104-1.7×108 cfu/g (aerobic mesophilic bacteria (AMB)), 1.5×10-2.7×107 cfu/g (coliform),0.0 to 6.1×101 (S. aureus) and 10-25 cfu/g (Pseudomonas spp.). Globally, 70% (21/30), 30%(09/30), 40% (12/30) and 6.67% (02/30) of the analyzed samples were unacceptable based on theload of AMB, coliform, S. aureus and Pseudomonas spp., respectively. Four coated skewers werecontaminated by Pseudomonas spp. About susceptibility to antimicrobial agents, 91.67% of S.aureus strains were resistant to ceftazidime and aztreonam, while a low resistance rate wasobserved for the other antibiotics. Pseudomonas spp strains, were resistant to cotrimoxazol (75%)but sensitive to the other antibiotics. These results highlight poor hygienic conditions of coatedskewers preparation in Ouagadougou. These practices contribute to their microbial contamination.Isolated bacteria showed a different level of resistance to the tested antibiotics. There is a need ofgood manufacturing practices to improve the hygienic quality of coated skewers.