Evaluation of antimicrobial effects of Zataria multiflora, Rosemarinus officinalis and Cuminum cyminum essential oils on the growth of Escherichia coli O157:H7
,
Abstract
Due to the increased resistance to antibiotics, identifying new compounds with fewer side effects is more important than usual medications in treatment of microorganisms. This study aimed to examine the antibacterial activity of Zataria multiflora, Rosemarinus officinalis and Cuminum cyminum essential oils on the growth of E. coli O157:H7 as one of the most common pathogens of food-borne diseases. Z. multiflora, R. officinalis and C. cyminum plants were dried and ground. Then the extraction of essential oil was performed by the hydrodistillation method. In order to examine the antibacterial effects, micro-broth dilution was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC). The disc diffusion method was applied to measure the inhibitory zone diameter. The data were analyzed by SPSS. Maximum diameter of inhibition zone (26.96 mm) was related to Z. multiflora which had a significant difference compared to other essential oils (p<0.001). Also the MIC and MBC of Z. multiflora essential oil were obtained as 3.125 mg/ml and 6.25 mg/ml, respectively. In this study, Zataria multiflora, Rosemarinus officinalis and Cuminum cyminum essential oils had significant antibacterial properties against E.coli O157:H7. As a result of these essential oils after completing studies, they can be a good alternative to chemical preservatives for storage of food.
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2. Garcia P, Rodriguez L, Rodriguez A, et al. Food biopreservation: promising strategies using bacteriocins, bacteriophages and endolysins. Trends Food Sci Technol 2010; 8: 373-382.
3. Badrei N, Gobin A, Dookeran S, et al. Consumer awareness and perception to food safety hazards in Trinidad, West Indies. Food Control 2006; 17:370-7.
4. Wang XW, Zhang L, Jin LQ, et al. Development and application of an oligonucleotide microarray for the detection of foodborne bacterial pathogens. Appl Microbiol Biotechnol 2007; 76(1):225-33.
5. Sarshar M, Doosti A, Jafari A, et al. Evaluation of oligonucleotide microarray technology for the detection of foodborne bacterial pathogens. J Microbial World 2009; 2: 73-80 [ in persian].
6. Headrick ML, Tollefson L. Food borne disease summary by food commodity. Vet Clin North Americ Food Animal Pract 1998; 14: 91-100.
7. Baldursson S, Karanis P. Waterborne transmission of protozoan parasites: review of worldwide outbreaks–an update 2004-2010. Water Res 2011; 45: 6603-14.
8. Momose Y, Hirayam K, Iton K. Effect of organic acids on inhibition of Escherichia coli O157:H7 colonization in gnotobiotic mice associated with infant intestinal microbiota. Antonie Leeu Wenhoek 2008; 93:141-149.
9. Chaucheyras-Durand F, Madic J, Dondin F, et al. Biotic and abiotic factors influencing in vitro growth of Escherichia coli O157:H7 in ruminant digestive contents. Appl Environ Microbiol 2006; 72: 4136-4142.
10. Tavakoli HR, Sarraf pour R, Samadi M. Water, Food and Bioterrorism. J Mil Med 2005; 7: 75-82 [ in persian].
11. Centers for Disease Control and Prevention. 2008-2012. Retrieved 2009-05-22.
12. Wang G, Clark CG, Rodgers FG. Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7serotype, and components of the Type 2 Shiga toxin family by multiplex PCR. J Clin Microbiol 2002; 40: 3613-19.
13. Mehdizadeh M, Eskandari S, Zavar M, et al. The Importance of Escherichia coli O157:H7 in Foodborn Infection. J. Kerman Univ Med Sci 2008; 15: 353-361 [in persian].
14. Jamshidi A, Bassami MR, Rasooli M. Isolation of Escherichia coli O157:H7 from ground beef samples collected from beef markets, using conventional culture and polymerase chain reaction in Mashhad, northeastern Iran. Iran J Vet Res 2008; 9: 72-76.
15. Takahashi M, Taguchi H, Yamaguchi H, et al. The effect of probiotic treatment with Clostridum butricum on enterohemorrhagic Escherichia coli O157:H7 infection in mice. FEMS Immunol Med Microbiol 2004; 41:219-226.
16. Shekarforoush SS, Nazer AHK, Firouzi, R et al. Effects of storage temperatures and essential oils of oregano and nutmeg on the growth and survival of Escherichia coli O157:H7 in barbecued chicken used in Iran. J Food Control 2007; 18: 1428-1433.
17. AAla F, Khodaveysi S, Baghdadi E, et al. The antifungal effect of Pistacia Atlantica subsp.Kurdica on the growth of Aspergillus parasiticus. J Res Health Sci 2015; 13: 79-84[in persian].
18. Hashemi A, Shams S, Barati M, et al. Antibacterial effects of metabolic extracts of Zataria multiflora ,Myrtus communis and peganum harmala on Pseudomonas aeruginosa producing ESBL. Arak Med Uni J 2011; 14: 104-112 [in persian].
19. Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 2004; 94: 223-253.
20. Campo JD, Amiot MJ, Nguyen-The C. Antimicrobial effect of rosemary extracts. J Food Protect 2000; 63: 1359-68.
21. Ozcan M. Antioxidant activities of rosemary, sage, and sumac extracts and their combinations on stability of natural peanut oil. J Med Food 2003; 6: 267-70.
22. Oroojalian F, Kasra-Kermanshahi R, Azizi MA, et al. Synergistic antibacterial activity of the essential oils from three medicinal plants against some important food-borne pathogens by microdilution method. Iranian J Med & Arom Plant 2010; 26: 133-46.
23. Gachkar L, Yadegari D, Rezaei MB, et al. Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chem 2007; 102: 898-904.
24. Mohammadi KH, Karim G, Hanifian SH, et al. Antimicrobial effect of Zataria multiflora Boiss. essential oil on Escherichia coli O157:H7 during manufacture and ripening of white brined cheese. J Food Hyg 2011; 1: 69-78[ in persian].
25. Malakootian M, Hatami B. Survey of Chemical Composition and Antibacterial Activity of Rosmarinus Officinalis Essential oils on Escherichia Coli and Its Kinetic. J Toloo-e-behdasht 2013; 12: 1-13 [in persian].
26. CLSI. Performance standards for antimicrobial susceptibility testing; 24th informational supplement. CLSI document M100-S24. Waye, PA: Clinical and Laboratory Standards Institute; 2014.
27. Demirci F, Guven K, Demirci B, et al. Antibacterial activity of two Phlomis essential oils against food pathogens. Food Control 2008; 19: 1159-1164.
28. Valero M, Salmeron M. Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth. Int J Food Microbiol 2003; 85: 73-81.
29. Vernozy‐Rozand C, Ray‐Gueniot S, Ragot C et al. Prevalence of Escherichia coli O157: H7 in industrial minced beef. Letter Appl Microbiol 2002; 35(1): 7-11.
30. Dallal MM, Mashkani MG, Yazdi MH, et al. Antibacterial effects of Rosmarinus officinalis on Methicillin-resistant Staphylococcus aureus isolated from patients and foods. Sci J Kurdistan Uni Med Sci 2011; 16: 73-80 [in persian].
31. Zandi H, Hajimohammadi B, Amiri Aet, al. Antibacterial Effects of (Mentha X Piperita L.) Hydroalcoholic Extract on the Six Food-Borne Pathogenic Bacteria. Toloo-e-behdasht 2016 ;15: 22-33 [in persian].
32. Ataee M, Hosseini H, Noori N, et al. Effect of Zataria multiflora boiss. essential oil on growth curve and shigatoxin 2 production of enterohemorrhagic escherichia coli O157: H7. J Med Plant 2013; 4: 62-71 [in persian].
33. Masoomi V, Tajik H, Moradi M, et al. Antimicrobial effects of Zataria multiflora boiss. essential oil nanoemulsion against Escherichia coli O157: H7. J Urmia Uni Med Sci 2016; 27: 608-17 [in persian].
34. Dallal MM, Bayat M, Yazdi MH, et al. Antimicrobial effect of Zataria multiflora on antibiotic-resistant Staphylococcus aureus strains isolated from food. Scientific J Kurdistan Uni Med Sci 2012; 17: 21-29 [in persian].
35. Yaghobzadeh N, Ownagh A, Mardani K, et al. Molecular identication, antibiotic resistance profile of shiga toxin-producing Escherichia coli(STEC) and antibacterial activity of Zataria multiflora boiss and Carum copticum essential oil against them. Urmia Med J 2011; 22: 262-269 [in persian].
36. Daneshmandi S, Soleimani N, Sattari M, et al. Evaluation of the drug synergistic and antibacterial effects of cuminum cyminum essential oil. Arak Med Uni J 2010; 13: 75-82 [in persian].
37. Bonyadian M, Karim G. Study of the effect of some volatile oils of herbs (pennyroyal, peppermint, tarragon, caraway seed and Thyme) against E.coli and S.aureus in broth media. J Vet Res 2002; 57: 81-83.
38. Motamedifar M, Hashemizadeh Z, Haghighati M, et al. Evaluation of Antibacterial Effects of Aqueous and Alcoholic Extracts of Cuminum cyminum L. Seeds on Some Pathogenic Bacteria. Sadra Med Sci J 2017; 3: 21-30 [in persian].
39. Suliman SK. Effect of crude methanolic extract of Cuminum cyminum on growth of some types of pathogenic bacteria. J Tikrit Uni Agri Sci 2009; 9: 611-615.
40. Haghighi F, Roudbar Mohammadi S, Soleimani N, et al. Evaluation of antifungal activity of essential oils of Thymus vulgaris, Petroselinum Crispum, Cuminum cyminum and Bunium persicum on candida albicans in comparison with Fluconazole. Pathobiol Res 2011; 14: 29-35 [in persian].
2. Garcia P, Rodriguez L, Rodriguez A, et al. Food biopreservation: promising strategies using bacteriocins, bacteriophages and endolysins. Trends Food Sci Technol 2010; 8: 373-382.
3. Badrei N, Gobin A, Dookeran S, et al. Consumer awareness and perception to food safety hazards in Trinidad, West Indies. Food Control 2006; 17:370-7.
4. Wang XW, Zhang L, Jin LQ, et al. Development and application of an oligonucleotide microarray for the detection of foodborne bacterial pathogens. Appl Microbiol Biotechnol 2007; 76(1):225-33.
5. Sarshar M, Doosti A, Jafari A, et al. Evaluation of oligonucleotide microarray technology for the detection of foodborne bacterial pathogens. J Microbial World 2009; 2: 73-80 [ in persian].
6. Headrick ML, Tollefson L. Food borne disease summary by food commodity. Vet Clin North Americ Food Animal Pract 1998; 14: 91-100.
7. Baldursson S, Karanis P. Waterborne transmission of protozoan parasites: review of worldwide outbreaks–an update 2004-2010. Water Res 2011; 45: 6603-14.
8. Momose Y, Hirayam K, Iton K. Effect of organic acids on inhibition of Escherichia coli O157:H7 colonization in gnotobiotic mice associated with infant intestinal microbiota. Antonie Leeu Wenhoek 2008; 93:141-149.
9. Chaucheyras-Durand F, Madic J, Dondin F, et al. Biotic and abiotic factors influencing in vitro growth of Escherichia coli O157:H7 in ruminant digestive contents. Appl Environ Microbiol 2006; 72: 4136-4142.
10. Tavakoli HR, Sarraf pour R, Samadi M. Water, Food and Bioterrorism. J Mil Med 2005; 7: 75-82 [ in persian].
11. Centers for Disease Control and Prevention. 2008-2012. Retrieved 2009-05-22.
12. Wang G, Clark CG, Rodgers FG. Detection in Escherichia coli of the genes encoding the major virulence factors, the genes defining the O157:H7serotype, and components of the Type 2 Shiga toxin family by multiplex PCR. J Clin Microbiol 2002; 40: 3613-19.
13. Mehdizadeh M, Eskandari S, Zavar M, et al. The Importance of Escherichia coli O157:H7 in Foodborn Infection. J. Kerman Univ Med Sci 2008; 15: 353-361 [in persian].
14. Jamshidi A, Bassami MR, Rasooli M. Isolation of Escherichia coli O157:H7 from ground beef samples collected from beef markets, using conventional culture and polymerase chain reaction in Mashhad, northeastern Iran. Iran J Vet Res 2008; 9: 72-76.
15. Takahashi M, Taguchi H, Yamaguchi H, et al. The effect of probiotic treatment with Clostridum butricum on enterohemorrhagic Escherichia coli O157:H7 infection in mice. FEMS Immunol Med Microbiol 2004; 41:219-226.
16. Shekarforoush SS, Nazer AHK, Firouzi, R et al. Effects of storage temperatures and essential oils of oregano and nutmeg on the growth and survival of Escherichia coli O157:H7 in barbecued chicken used in Iran. J Food Control 2007; 18: 1428-1433.
17. AAla F, Khodaveysi S, Baghdadi E, et al. The antifungal effect of Pistacia Atlantica subsp.Kurdica on the growth of Aspergillus parasiticus. J Res Health Sci 2015; 13: 79-84[in persian].
18. Hashemi A, Shams S, Barati M, et al. Antibacterial effects of metabolic extracts of Zataria multiflora ,Myrtus communis and peganum harmala on Pseudomonas aeruginosa producing ESBL. Arak Med Uni J 2011; 14: 104-112 [in persian].
19. Burt S. Essential oils: their antibacterial properties and potential applications in foods—a review. Int J Food Microbiol 2004; 94: 223-253.
20. Campo JD, Amiot MJ, Nguyen-The C. Antimicrobial effect of rosemary extracts. J Food Protect 2000; 63: 1359-68.
21. Ozcan M. Antioxidant activities of rosemary, sage, and sumac extracts and their combinations on stability of natural peanut oil. J Med Food 2003; 6: 267-70.
22. Oroojalian F, Kasra-Kermanshahi R, Azizi MA, et al. Synergistic antibacterial activity of the essential oils from three medicinal plants against some important food-borne pathogens by microdilution method. Iranian J Med & Arom Plant 2010; 26: 133-46.
23. Gachkar L, Yadegari D, Rezaei MB, et al. Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chem 2007; 102: 898-904.
24. Mohammadi KH, Karim G, Hanifian SH, et al. Antimicrobial effect of Zataria multiflora Boiss. essential oil on Escherichia coli O157:H7 during manufacture and ripening of white brined cheese. J Food Hyg 2011; 1: 69-78[ in persian].
25. Malakootian M, Hatami B. Survey of Chemical Composition and Antibacterial Activity of Rosmarinus Officinalis Essential oils on Escherichia Coli and Its Kinetic. J Toloo-e-behdasht 2013; 12: 1-13 [in persian].
26. CLSI. Performance standards for antimicrobial susceptibility testing; 24th informational supplement. CLSI document M100-S24. Waye, PA: Clinical and Laboratory Standards Institute; 2014.
27. Demirci F, Guven K, Demirci B, et al. Antibacterial activity of two Phlomis essential oils against food pathogens. Food Control 2008; 19: 1159-1164.
28. Valero M, Salmeron M. Antibacterial activity of 11 essential oils against Bacillus cereus in tyndallized carrot broth. Int J Food Microbiol 2003; 85: 73-81.
29. Vernozy‐Rozand C, Ray‐Gueniot S, Ragot C et al. Prevalence of Escherichia coli O157: H7 in industrial minced beef. Letter Appl Microbiol 2002; 35(1): 7-11.
30. Dallal MM, Mashkani MG, Yazdi MH, et al. Antibacterial effects of Rosmarinus officinalis on Methicillin-resistant Staphylococcus aureus isolated from patients and foods. Sci J Kurdistan Uni Med Sci 2011; 16: 73-80 [in persian].
31. Zandi H, Hajimohammadi B, Amiri Aet, al. Antibacterial Effects of (Mentha X Piperita L.) Hydroalcoholic Extract on the Six Food-Borne Pathogenic Bacteria. Toloo-e-behdasht 2016 ;15: 22-33 [in persian].
32. Ataee M, Hosseini H, Noori N, et al. Effect of Zataria multiflora boiss. essential oil on growth curve and shigatoxin 2 production of enterohemorrhagic escherichia coli O157: H7. J Med Plant 2013; 4: 62-71 [in persian].
33. Masoomi V, Tajik H, Moradi M, et al. Antimicrobial effects of Zataria multiflora boiss. essential oil nanoemulsion against Escherichia coli O157: H7. J Urmia Uni Med Sci 2016; 27: 608-17 [in persian].
34. Dallal MM, Bayat M, Yazdi MH, et al. Antimicrobial effect of Zataria multiflora on antibiotic-resistant Staphylococcus aureus strains isolated from food. Scientific J Kurdistan Uni Med Sci 2012; 17: 21-29 [in persian].
35. Yaghobzadeh N, Ownagh A, Mardani K, et al. Molecular identication, antibiotic resistance profile of shiga toxin-producing Escherichia coli(STEC) and antibacterial activity of Zataria multiflora boiss and Carum copticum essential oil against them. Urmia Med J 2011; 22: 262-269 [in persian].
36. Daneshmandi S, Soleimani N, Sattari M, et al. Evaluation of the drug synergistic and antibacterial effects of cuminum cyminum essential oil. Arak Med Uni J 2010; 13: 75-82 [in persian].
37. Bonyadian M, Karim G. Study of the effect of some volatile oils of herbs (pennyroyal, peppermint, tarragon, caraway seed and Thyme) against E.coli and S.aureus in broth media. J Vet Res 2002; 57: 81-83.
38. Motamedifar M, Hashemizadeh Z, Haghighati M, et al. Evaluation of Antibacterial Effects of Aqueous and Alcoholic Extracts of Cuminum cyminum L. Seeds on Some Pathogenic Bacteria. Sadra Med Sci J 2017; 3: 21-30 [in persian].
39. Suliman SK. Effect of crude methanolic extract of Cuminum cyminum on growth of some types of pathogenic bacteria. J Tikrit Uni Agri Sci 2009; 9: 611-615.
40. Haghighi F, Roudbar Mohammadi S, Soleimani N, et al. Evaluation of antifungal activity of essential oils of Thymus vulgaris, Petroselinum Crispum, Cuminum cyminum and Bunium persicum on candida albicans in comparison with Fluconazole. Pathobiol Res 2011; 14: 29-35 [in persian].
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| Issue | Vol 4 No 3/4 (2018): Summer/Autumn | |
| Section | Original Article(s) | |
| Keywords | ||
| Essential oil, Zataria multiflora, Rosemarinus officinalis, Cuminum cyminum, Antimicrobial effecets | ||
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How to Cite
1.
Vaziri S, Hamzeh pour S, Dalvand M. Evaluation of antimicrobial effects of Zataria multiflora, Rosemarinus officinalis and Cuminum cyminum essential oils on the growth of Escherichia coli O157:H7. J Food Safe & Hyg. 2019;4(3/4).
