Sub-acute oral toxicity evaluation of aqueous extract of bamboo shoots ( Dendrocalamus hamiltonii) in Balb/c mice

  • Harjit Kaur Bajwa Panjab University, Chandigarh
  • Oinam Santosh
  • Ashwani Koul
  • Madho Singh Bisht
  • Chongtham Nirmala Mail
Bamboo shoots, Histopathology, Sub-acute toxicity, Lipid peroxidation, Antioxidant enzymes


Bamboo shoot (Dendrocalamus hamiltonii), the young tender and immature culm is being considered as a complete health food due to its high nutritional and bioactive content. Although, freshly harvested bamboo shoots are nutritionally richer; anti-nutritional factors and toxicity in fresh shoots used for consumption are a matter of concern. This study was conducted to evaluate the sub-acute oral toxicity of aqueous extract in shoots of Dendrocalamus hamiltonii. The bamboo shoot extract was administered orally to Balb/c mice at doses of 400, 800 and 1600 mg/kg, bodyweight for 28 days. Results revealed a significant enhancement in the level of reduced glutathione and superoxide dismutase accompanied by a significant reduction in the level of lipid peroxidation. A dose-dependent increase was observed in the activity of glutathione peroxidase and glutathione reductase while a marked reduction was seen in the activity of enzyme catalase. None of any mortality and behavioral changes was observed. At higher dose (1600 mg/kg, body weight) the level of serum creatinine, serum glutamic pyruvic transaminase and lactate dehydrogenase increased significantly, however, the histo-architecture of liver and kidney was normal. Therefore, it is concluded that the medium-term oral administration of the bamboo shoot extract for 28 days does not cause toxicity.

Author Biographies

Oinam Santosh
Department of Botany, Panjab University, Chandigarh
Ashwani Koul
Professor Department of Biophysics Panjab University, Chandigarh
Madho Singh Bisht
Professor Department of Environmental Sciences NEHU, Shillong
Chongtham Nirmala
Professor Botany Department Panjab University, Chandigarh
How to Cite
Bajwa H, Santosh O, Koul A, Bisht M, Nirmala C. Sub-acute oral toxicity evaluation of aqueous extract of bamboo shoots ( Dendrocalamus hamiltonii) in Balb/c mice. J Food Safe & Hyg. 5(3).
Original Article(s)