Determination of Aflatoxin M1 in whey powder by HPLC and ELISA methods
Abstract
Whey powder is one of the dairy products that is used widely in the food industry. Therefore, it is necessary to make sure that it is healthy and is not contaminated with various toxins such as Aflatoxin M1, in order to protect the health of the consumers. Producing Whey powder from contaminated milk (with Aflatoxin M1) results in the products containing Aflatoxin M1. This study was conducted to determine the contamination of whey powder to Aflatoxin M1 in two domestic factories. Whey powder samples were collected during 8 months from December 2014 to July 2015. Regarding the Aflatoxin M1, the samples were evaluated by ELISA. Additionally, 26 samples were also analyzed by HPLC-FLD method. According to ELISA method, the contamination level of 44 samples (52.4%) was less than the permitted value (1000 ppt) and 40 samples (47.6%) were higher than the maximum tolerance limit recommended by Iranian national standard (Number: 5925). The average concentration of toxin in the studied samples was 1100.2±734.8 (ppt). The range of changes in concentration was between 200 and 7000 ppt. In the current study, seasonal impact on the level of Aflatoxin M1 was not significant among the samples. The results of HPLC and ELISA methods did not show significant differences in the detection of Aflatoxin M1 contamination. Seasonal impact on the level of Aflatoxin M1 was not significant among the samples. Contamination of whey powder with Aflatoxin M1 is harmful to human health.Prandini A, Tansini G, Sigolo S, et al. On the occurrence of aflatoxin M1 in milk and dairy products. Food and Chemical Toxicology 2009;47(5):984-91.
Azizi IG, Rouhi S. The comparison of total fumonisin and total aflatoxin levels in biscuit and cookie samples in Babol City, Northern Iran. Iranian journal of public health 2013;42(4):422.
Giovati L, Magliani W, Ciociola T, et al. AFM1 in milk: physical, biological, and prophylactic methods to mitigate contamination. Toxins 2015;7(10):4330-49.
Ricordy R, Cacci, E, et al. Review in food and nutrition toxicity. ed. 4th, editor. London: CRC Press; 2005:213-231
Anttila A, Bhat RV, Bond JA, et al. IARC monographs on the evaluation of carcinogenic risks to humans: some traditional herbal medicines, some mycotoxins, naphthalene and styrene. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans 2002;82.
Mendonc C¸ Venaˆncio A. Fate of aflatoxin M1 in cheese whey processing. Journal of the Science of Food and Agriculture 2005; 85(12):2067-70
Unusan N. Occurrence of aflatoxin M1 in UHT milk in Turkey. Food and Chemical Toxicology 2006;44(11):1897-900.
European Commission. Commission Regulation 2001/466/EC of 8 March 2001 setting maximum levels for certain contaminants in foodstuffs (text with EEA relevance).Official Journal of the European Union 2001;77: 1-13.
Iranian National Standardization Organization 6959 2nd. Revision 2016.
Kos JJ, Hajnal EJ, et al. Comparison of ELISA, HPLC-FLD and HPLC-MS/MS methods for determination of aflatoxin M1 in natural contaminated milk samples. Acta Chimica Slovenica 2016;63(4):747-56.
Walfish S. Analytical methods: a statistical perspective on the ICH Q2A and Q2B guidelines for validation of analytical methods. BioPharm International 2006;19(12):1-6.
Galvano F, Galofaro V, Galvano G. Occurrence and stability of aflatoxin M1 in milk and milk products: a worldwide review. Journal of Food protection 1996;59(10):1079-90.
Kamkar A. Detection of Aflatoxin M1 in UHT milk samples by ELISA. J Vet Res 2008;63(2):7-12.
Rahimi E, Mohammadhosseini Anari M, et al. Aflatoxin M1 in pasteurized milk and white cheese in Ahvaz, Iran. Global veterinaria 2012;9(4):384-7.
Kamkar A. Study on the contamination of UHT milks with aflatoxin M1 in the city of Tehran Journal of the Faculty of Veterinary Medicine. University of Tehran 2002;57(4):5-8.
Kamkar A, Khaniki GRJ, Alavi S. Occurrence of aflatoxin M1 in raw milk produced in Ardebil of Iran 2011; 8(2); 123-128
Fallah AA. Aflatoxin M1 contamination in dairy products marketed in Iran during winter and summer. Food control 2010;21(11):1478-81.
Mulunda M, Ngoma L, Nyirenda M, Motsei L, Bakunzi F. A Decade of Aflatoxin M1 Surveillance in Milk and Dairy Products in Developing Countries (2001-2011): A Review. Mycotoxin and Food safety in developing countries: InTech 2013.
Markaki P, Melissari E. Occurrence of aflatoxin M1 in commercial pasteurized milk determined with ELISA and HPLC. Food Additives & Contaminants 1997;14(5):451-6.
Sadeghi E, Almasi A, et al. The evaluation of aflatoxin M1 level in collected raw milk for pasteurized dairy factories of Kermanshah 2013; 15(3): 26 -29
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| Issue | Vol 4 No 1/2 (2018): Winter/Spring | |
| Section | Original Article(s) | |
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| Aflatoxins HPLC ELISA Whey powder | ||
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