Assessment of total protein and soy protein contents in some heated meat products in Tehran, Iran
AbstractHeated meat products are emulsion which have various nutrition materials such as meat as an animal protein and soya as a plant protein. The nutritional value of meat proteins is very high than soya protein but the meat is more expensive than soya which the producers substitute the meat with soya. This study was assessed the soy protein and the total protein contents in some heated meat products collected from food stores in Tehran city of Iran. Twenty samples of heated meat products with 40%, 55% and 70% of meat were randomly collected from food stores. The heated meat products samples were transferred to the food analysis lab. The total protein was determined by the macro Kjeldahl method after sample preparation and homogenization. Also, the soy protein content in samples was measured by using the enzyme-linked immunosorbent assay (ELISA) method. Results showed that 4 samples of heated meat products had less total protein content than the standard limit and 16 samples were in accordance with Iranian national standard. Soy protein content in 3 from 8 samples of heated meat products with 40% meat was higher than the standard limit and the others placed in the standard limit (approximate with 4% soy protein). Also, Soy protein content in 6 from 8 samples of heated meat products with 55% meat was higher than the standard limit and only 2 samples were in accordance with the standard limit. All samples of heated meat products with 70% meat set in a standard limit. It was concluded that some heated meat products do not correspond with the Iranian national standard range. The food quality control lab requires doing attention and sensation for correct formulation according to national standard measures.
Rokni N. Meat Science and Technology. University of Tehran Press. 2006.
Joussen FW, Voortman G, Debaaij AJ. Detection of wheat gluten, whey protein, casein, ovalbumin and soy protein in heated meat products by electrophoresis blotting and immuno peroxidase staining. J Agric Food Chem 1987; 35: 563 – 567.
Belloqu J, Garcia MC, Torre M, Martin ML. Analysis of soybean proteins in meat products. A review critical review in Food Science and Nutrition 2002; 42: 507 – 532.
ISIRI. Sausages and calbas: Specifications and examination methods. Institute of Standards and Industrial Research of Iran(ISIRI). 2006; No.2303.
Shahrasbi H, Naseri A. Nutritional value and practical methods for chemical and hygienic control in some of meat products in Iran. Publication of Jahade Daneshgahi, Tehran, Iran. 1985.
Jansen JJ, Kardinaal AF, Huijbers G, Vlieg – Boerstra BJ, Martens BP, Ockhuizen T. Prevalence of food allergy and intolerance in the adult Dutch population. J Allergy Clinic Immunol 1994; 93: 446 – 456.
Zarkados M, Scott WF, Salminen J, Pony AH. Common Allergenic foods and their labeling in Canada. Candian Journal of Allergy and Clinical Immunology( 999; 4: 118 – 141.
Flint C Meech M.V. Quantitative determination of textured soy protein by a stereological technique. Analyst 1978; 103:252.
Castro F, Garcia MC, Rodriguez R, Marina ML. Determination of soybean protein in commercial heat – processed meat products prepared with chicken, beef or complex mixtures of meats from different species. Food Chemistry 2007; 100: 468 – 476.
Jahed Khaniki, Gh R, Rokni, N. Evaluation of Histological methods in detection of unpermitted tissues in heated meat products in Iran. Pajouhesh va Sazandegi 2003: 62.
AOACSoy protein in raw and heat – processed meat products. Enzyme – Linked Immune Sorbent Assay, AOAC official method 2004; 998:10.
Luthy J. Detection strategies for food authenticity and genetically modifiers foods. Food Control 1999; 10: 359- 361.
Cota M, Vallejo B, Calderon AM, Gonzalez AF. Immunochemical detection of fraudulent substitution of pork chorizo with soy protein. Food Science and Technology International 1998; 4: 257 – 262.
Medina, M.B. Extraction and quantization of soya protein in sausages by ELISA. Journal of Agricultural and Food Chemistry 1988; 36: 766-771.
Macedo SA, Shimokmak MAJ, Yamamot YY. Textured soy protein quantification in commercial Hamburger. Journal of Food Composition Analysis 1999; 14, 469 – 478.
Koppelman SJ, akemond CMM, Vlooswijk R, Hefle SL. Detection of soy proteins in processed foods. Literature overview and new experimental work. Journal of AOAC International 2004; 87: 1389 – 1407.
Montserrat E, Beatriz H, Juan M. Validation of end – point and real – time PCR methods for the rapid detection of soy allergen in processed products. Food Additive and Contaminants 2010; 27: 426- 432.
Asensio L, Gonzalez I, Garcia T, Martin R. Determination of food authenticity by Enzyme – Linked Immune Sorbent Assay (ELISA). Journal of Food Protection (2007); 66: 869-886.
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