One-step indirect competitive ELISA development for dexamethasone determination in chicken
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Abstract
Dexamethasone (DEX) is a potent glucocorticoid that was widely employed in livestock production. In the present study, a one-step indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed for the determination of DEX residue in animal-origin food. With this aim, hapten DEX-3c was firstly synthesized, conjugated to carrier protein, and then used as immunogen in rabbits. Polyclonal antibody was subsequently obtained and characterized. The half-maximal inhibition concentrations (IC50) and the limit of detection (LOD, calculated as IC20) of the icELISA for DEX were approximately 0.061 and 0.015 ng/mL, respectively. The antibody exhibited significantly cross-reactivity with some of DEX structural analogs. The average recoveries and coefficients of variation (CVs) of DEX from fortified samples by icELISA were in a range of 83.3- 114.2% and 5.6-9.5%, respectively. The LOD of this assay for DEX in chicken muscle and liver extracts was 0.3 μg/kg and 0.5 μg/kg, respectively.Rebuffat AG, Tam S, Nawrocki AR, et al. The 11-ketosteroid 11-ketodexamethasone is a glucocorticoid receptor agonist. Mol Cell Endocrinol 2004; 214 :27-37.
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| Issue | Vol 2 No 3/4 (2016): Summer/Autumn | |
| Section | Original Article(s) | |
| Keywords | ||
| Dexamethasone Broad-specificity polyclonal antibody (pAb) One-step icELISA Chicken muscle Chicken liver | ||
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