Journal of Food Safety and Hygiene 2016. 2(3/4):84-89.

The effect of ethanolic extract of walnut thin shell on the growth of Aspergillus spp.
Ehsan Haghi, Sassan Rezaie, Ebrahim Molaee Aghaee, Parisa Sadighara, Fereshteh Ahmadi


Aspergillus spp. are the most important fungi for production of Aflatoxin. They are heat resistant and can be easily adapted to the environment. The toxin of these fungi can enter the bodies through the food chain and causes major health problems. It can also make serious troubles in many fields such as food industry, animal husbandry and economic. Some studies have investigated the effects of herbal and plant extracts to reduce the growth of Aflatoxin resulting inhibition of toxin production. Several benefits have been addressed for different parts of walnut. Thin shell around the walnut is full of phenolic and antioxidant compounds that may have the effect on Aspergillus flavus and Aspergillus fumigates. This study explored the effects of ethanol extract obtained from walnut thin shells on standard and isolated species of Aspergillus flavus and Aspergillus fumigatus. The extract dilutions of 500 to 3.75 mg per ml were exposure to the desired fungi using the broth dilution method. minimum inhibitory concentrations (MI) of fungi were determined and compared with the effects of Nystatin and fluconazole. The minimum inhibitory concentration for Aspergillus fumigatus and Aspergillus flavus were 15 mg/ml and 61.5 mg/ml respectively. walnut Thin shells has antifungal activity and could inhibit the growth of Aspergillus flavus and Aspergillus fumigatus.


walnut shell extract; Aspergillus flavus; Aspergillus fumigatus; MIC

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