Tehran University of Medical Sciences Journal of Food Safety and Hygiene 2476-3241 1 1 2015 10 05 35 38 EN Parviz Mohajeri Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran Hassan Abassi Department of Pharmacology, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran Nabi Shariatifar Department of Environment Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ayub Ebadi-Fathabad Department of Environment Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2015 10 05 2015 10 05 Food-borne  pathogenic  is a group of micro -organis ms that caus e food -borne illnes s thus ,  the  res earch  for  finding  effective  drugs  agains t  this  infection  is  neces s ary. Staphylococcus  aureus is the major caus e of food -borne and nos ocomial infection in las t decade  and  methicillin  res is tant  S.  aureus  (MRSA)  has  emerged  as  a major clinical problem.  The  aim of the pres ent s tudy was to compare different  phenotypic  methods with genotypic method by polymeras e chain reaction (PCR) for the detection of MRSA s trains .   Sens itivity   to   methicillin   was   als o   inves tigated   by   oxacillin,   methicillin, cefotetan,  cefoxitin,  cefmetazole  dis ks ,  the  data  tab, and  dis k diffus ion  method  and oxacillin   s trip.   Minimum   inhibitory   concentration    (MIC)   of   thes e   is olates   was determined  by us ing micro-broth dilution. A total of 186 is olates of S. aureus with PCR method  (gold  s tandard)  were detected. About 95 is olates  were methicillin -s ens itive S. aureus , and  91 is olates  were  MRSA.  Among  the  diagnos tic methods  s tudied, micro - broth  dilution  and  cefoxitin  dis k  had  the  mos t  s pecificity  with  98.9%  and  94.7%, res pectively. The s ens itivity of them was 100.0% and 98.9%, res pectively. Furthermore, the  concordance  with  PCR  was  98.9%  and  93.6%,  res pectively.  The  cefotetan  and cefmetazole  dis ks  had  the  lowes t  concordance  with  the  res ults  of PCR. Due  to  the neces s ity   of   us ing   s imple,  reliable   and   low-cos t  methods   in   routine   diagnos tic laboratories  it  s eems  us e of cefoxitin  dis k s till be cons idered as one of recommended methods for detecting MRSA is olates . https://jfsh.tums.ac.ir/index.php/jfsh/article/view/8 https://jfsh.tums.ac.ir/index.php/jfsh/article/download/8/8