Tehran University of Medical Sciences Journal of Food Safety and Hygiene 2476-3241 9 3 2023 09 30 197 206 Eniola Oni Federal University of Agriculture, Abeokuta, Nigeria Nathaniel Olorunsogo Oni Nigeria Julius Bamidele Nigeria Odunayo Anthony Taiwo Nigeria Olubukola Blessing Oyetibo Nigeria Amina Omodolapo Badmos Nigeria Tolulope Modupeolu Adeleye Nigeria Mariam Omobolanle Olatunbosun Nigeria Flora Oluwafemi Nigeria 2023 08 18 2023 10 05 Contamination of locally formulated herbs with aflatoxins and pathogenic organisms poses major health concerns to humans and animals, especially in recent times when herbal mixtures are on the rise. This study aimed to determine the herbs' microbial profile and aflatoxin level. Two different herbal medicines (malaria and typhoid; each prepared with water and alcohol) were obtained at Itoku market, Ogun-state, Nigeria. The samples were isolated using the serial dilution technique and isolates were identified morphologically. Aflatoxin quantification was done on the herbal samples using High-Performance Liquid Chromatography (HPLC). The viable bacteria count ranged from 1.0×105 cfu/g to 20.0×105 cfu/mL with the typhoid herbs prepared with water recording the highest count. The microorganisms obtained in the herbs were confirmed as, Escherichia coli, Staphylococcus aureus, Micrococcus luteus, Salmonella sp, Proteus sp, Aspergillus flavus, Penicillium chrysogenum, Saccharomyces cerevisae and Fusarium sp. Typhoid herbs prepared with water showed high aflatoxin detection limits of 7.60 μg/mL. The result showed that the locally formulated herbs were highly contaminated with microorganisms and that consumption of the locally formulated herbs with aflatoxin could cause aflatoxicosis. https://jfsh.tums.ac.ir/index.php/jfsh/article/view/463 https://jfsh.tums.ac.ir/index.php/jfsh/article/download/463/225