Tehran University of Medical Sciences Journal of Food Safety and Hygiene 2476-3241 1 1 2015 10 05 13 17 EN Naiema Vakili-Saatloo Department of Environmental Health Engineering, Division of Food Safety and Hygiene, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Parisa Sadighara Department of Environmental Health Engineering, Division of Food Safety and Hygiene, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Gholamreza Jahed-Khaniki Department of Environmental Health Engineering, Division of Food Safety and Hygiene, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Najmeh Ebrahimi Department of Environmental Health Engineering, Division of Food Safety and Hygiene, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Samaneh Nabizadeh Department of Environmental Health Engineering, Division of Food Safety and Hygiene, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2015 10 05 2015 10 05 Food additives are the most common substances that are used in food industries. Different bioassays are required for the determination of mixtures of food additives. In this study, the toxicity of three common synthetic food dyes such as Carmoisine, Sunset Yellow, and Brilliant Blue in combination of aspartame was screened against the larvae of Artemia salina. At first, lethal dose50 (LD50) of each food additives was calculated and then the combinations of them were assayed. Results showed that the  toxicity of Sunset Yellow with aspartame (LD50  = 42 µg/ml) is more than other dyes and the combination of Carmoisine with aspartame (LD50 = 88.25) is the safer than others. https://jfsh.tums.ac.ir/index.php/jfsh/article/view/3 https://jfsh.tums.ac.ir/index.php/jfsh/article/download/3/3