Tehran University of Medical Sciences Journal of Food Safety and Hygiene 2476-3241 6 2 2021 04 30 67 81 Adriana Morales Gomez College of Veterinary Medicine, Cornell University Elizabeth Nunez College of Veterinary Medicine, Cornell University Patrick McDonough College of Veterinary Medicicine, Cornell University Yung-Fu Chang College of Veterinary Medicine, Cornell University Hussni Mohammed College of Veterinary Medicine, Cornell University 2020 12 06 2021 04 22 Foodborne pathogens represent one of the major challenges to health systems around the world. This risk is exacerbated by the presence of antimicrobial-resistant (AMR) pathogens. Knowledge on the presence of these pathogens in the food supply chain would help in establishing intervention strategies to mitigate their risk. The objective of this study was to detect AMR among serotypes of Escherichia coli (E. coli) food adulterants serotypes of E. coli in the food supply chain and among isolates from gastroenteritis cases. E. coli isolates recovered form animals, meet processing plants, retail, and humans were examined for the presence of AMR using phenotypic and genotypic approaches. AMR to aminoglycosides, β-lactams, and tetracycline were detected in all isolates recovered from these sources at different levels. Similarly, presence of the bla-Tem, bla-SHV, aadA, and strAB genes were detected in isolates from these sources but there was no significant correlation between the genetic detection and phenotypic expression AMR. https://jfsh.tums.ac.ir/index.php/jfsh/article/view/283 https://jfsh.tums.ac.ir/index.php/jfsh/article/download/283/142