Tehran University of Medical Sciences Journal of Food Safety and Hygiene 2476-3241 5 4 2020 12 26 230 236 EN Inam Ullah Department of Microbiology and Immunology Hebei Medical University Shijiajhuang China AND Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan. Hajra Khurshid Department of Biosciences COMSATS University Islamabad Pakistan Nadeem Ullah Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan. AND Republic Department of China Pathogen Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Israr Aziz Department of Biosciences COMSATS University Islamabad Pakistan Muhammad Junaid Khan Department of Microbiology Abdul Wali Khan University Mardan Pakistan Basit Ali Khan Department of Microbiology Government College University Lahore Pakistan Junaid Ahmad Department of Microbiology ,Hazara University, Mansehra, Pakistan Zahid Ullah Departemt of Microbiology, Hazara University, Mansehra, Pakistan 2020 09 30 2020 10 30 The campylobacter genus of bacteria is important in public health as it comprises many species causing diarrhea in humans. Poultry and its products are recognized as vital causes of campylobacteriosis in humans. For bacterial food-borne diseases, campylobacter is considered as the leading cause. Higher prevalence has been reported in developed countries. Our study was a cross-sectional study directed to determine the prevalence of campylobacter species in retail broiler meat in the Bannu district of Khyber Pakhtunkhwa, Pakistan, from January 2018 to June 2018. A total of 200 poultry meat samples were collected from four different areas of district Bannu Khyber Pakhtunkhwa province of Pakistan that includes lakki gate, tanchi bazar, Bannu Township and mangal milla. Mueller-Hinton medium was used for the disc diffusion method to determine antibiotic resistance of campylobacter species. Amongst 200 broiler meat samples, 60 (30%) samples were found positive for campylobacter species. The highest prevalence was observed in samples from Bannu Township (50%) while the lowest prevalence (12%) was observed in samples from Mangal milla broiler meat samples. Amongst different types of meat samples, the highest prevalence was found in thigh meat (46%), while the lowest prevalence was observed in the cloacal swab (20%). The highest resistance was observed against Amoxicillin (AMX) 80% while the resistance observed against other antibiotics were Ampicillin (AMP) 70%, Tetracycline (TET) 65%, Sulphamethoxazole + Trimethoprim (SXT) 60%, Chloramphenicol (CHL) 56.66%, Clarithromycin (CLR) 50%, Streptomycin (STR) 40%, Gentamycin (GEN) 36.66%, Ofloxacin (OFX) 20%, Ciprofloxacin (CIP) 15%, Levofloxacin (LEV) 15% and Azithromycin (AZM) 10%. The lowest resistance was observed against Ceftriaxone (CRO) 5%. Our Study concludes that campylobacter species are prevalent highly in district Bannu, and it might be a hazard to public health. https://jfsh.tums.ac.ir/index.php/jfsh/article/view/273 https://jfsh.tums.ac.ir/index.php/jfsh/article/download/273/123