Tehran University of Medical Sciences Journal of Food Safety and Hygiene 2476-3241 6 1 2021 03 10 22 27 Hoda Ashari Department of Drug and food control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Naficeh Sadeghi Department of Drug and food control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Mohammad Reza Oveisi Department of Drug and food control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Mannan Hajimahmoudi Drug and Food Control Department, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, IranFood and Drug Administration, Tehran University of Medical Sciences, Tehran, Iran 2020 07 18 2021 03 08 This study was designed to measure and compare the total phenols, flavonoids and vitamin C contents in four orange juice brands which are commercially available in Iran. Total phenols, flavonoids and ascorbic acid content of 100 samples from four different commercial brands were evaluated by the spectrometric method. The concentration of total phenol in commercial orange juice samples was between 28.39 and 114.20 mg gallic acid equivalent per liter (mg GAE/L). The measured range of total flavonoids was from 12.53 to 32.62 mg quercetin equivalent per liter (mg QE/L) and the content of ascorbic acid in the samples was between 29.95 and 93.08 mg/L. The results showed a significant difference between the four brand`s total phenols, flavonoids and vitamin C level (P< 0.05). According to the found variation among different studied brands, setting a determined amount for the measured parameters is suggested. https://jfsh.tums.ac.ir/index.php/jfsh/article/view/267 https://jfsh.tums.ac.ir/index.php/jfsh/article/download/267/136