Tehran University of Medical Sciences Journal of Food Safety and Hygiene 2476-3241 1 2 2015 12 08 63 68 EN Samaneh Nabizadeh Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Nabi shariatifar Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Shahram Shoeibi Food and Drug Laboratory Research Center, Food and Drug Organization, Ministry of Health and Medical Education, Tehran, Iran Gholamreza Jahed-Khaniki Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Ramin Nabizadeh-Nodeh Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Esmail Shokoohi Department of Basic Science,Faculty of Science, Islamic Azad University of Ahar Branch, Ahar, Iran 2015 12 07 2015 12 07 Vegetable oils, especially olive oil, are used as healthy fats in modern nutrition. The study aim was to optimize and validate a methodology for the simultaneous analysis of aflatoxins B1, B2, G1 and G2 in olive oil by high performance liquid chromatography-fluorescence detector (HPLC-FLD). The procedure of the validation for the determination of aflatoxin B1, B2, G1 and G2 in traditional olive oil was performed. The recovery of AFS (B1, B2, G1 and G2) in three spiking levels was in range of 84.45-109%. The repeatability of measurements, represented by the standard deviation (RSDr) was 8.11 %,8.05%, and 3.76 % at the spiking levels of 0.5ng/g, 2 ng/g and 6 ng/g,respectively. The limit of quantification (LOQs) and limit of detection (LOD)were 0.5ng/g and 0.16 ng/g. The results of validation parameters show that he procedure is suitable and simple for the determination of aflatoxins B1,B2, G1 and G2 in traditional olive oil and can be implemented for the routine analysis.     https://jfsh.tums.ac.ir/index.php/jfsh/article/view/17 https://jfsh.tums.ac.ir/index.php/jfsh/article/download/17/19